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- Title
Quantitative Methods for Metabolite Analysis in Metabolic Engineering.
- Authors
Ahn, Cheeyoon; Lee, Min-Kyung; Jung, Cheulhee
- Abstract
We present a brief overview of metabolic engineering, depicting the necessity of exploiting microorganisms for obtaining desired metabolites and the difficulty of metabolic pathway optimization under numerous conditions. The advantages, limitations, and examples of conventional quantitative analytical methods that focus on accuracy but have low throughput rates are presented. We have also described in vivo analytical methods with high-throughput rates, which indirectly compare the yield of the reporter protein. Additionally, we have explained a few considerations for engineering in vivo analytical methods. This review also highlights the current challenges faced by the analytical methods in the metabolic engineering.
- Subjects
ENGINEERING mathematics; QUANTITATIVE research; METHODS engineering; TRANSCRIPTION factors; TISSUE engineering
- Publication
Biotechnology & Bioprocess Engineering, 2023, Vol 28, Issue 6, p949
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-022-0200-z