We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of a Major Yolk Protein as an Allergen in Sea Urchin Roe.
- Authors
Yamasaki, Ayako; Higaki, Hiromi; Nakashima, Keiko; Yamamoto, Osamu; Kyaw Zaw Hein; Takahashi, Hitoshi; Chinuki, Yuko; Morita, Eishin
- Abstract
Anaphylaxis after eating sea urchin roe has been reported. However, its major allergens have not yet been identified. The aim of this study was to identify the major allergens of sea urchin roe. Proteins of sea urchin roe were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis (2-DE). An immunoglobulin (Ig)E-binding protein was detected by immunoblotting using the patient's serum. An allergen isolated from 2DE-gel was identified by peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Immunoblot analysis of sea urchin extracts showed that a 160-kDa protein at p1 6-7 was recognized by the patient's IgE. Peptide mass fingerprint analysis revealed that the protein was the major yolk protein (152 kDa, p1 6.9) of sea urchins. The results show that a major allergen of sea urchin roe is the major yolk protein.
- Subjects
ALLERGENS; SEA urchins; ELECTROPHORESIS; IMMUNOBLOTTING; PEPTIDES; IONIZATION (Atomic physics)
- Publication
Acta Dermato-Venereologica, 2010, Vol 90, Issue 3, p235
- ISSN
0001-5555
- Publication type
Article
- DOI
10.2340/00015555-0783