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- Title
Development of a whole‐cell biosensor for ethylene oxide and ethylene.
- Authors
Moratti, Claudia F.; Yang, Sui Nin Nicholas; Scott, Colin; Coleman, Nicholas V.
- Abstract
Ethylene and ethylene oxide are widely used in the chemical industry, and ethylene is also important for its role in fruit ripening. Better sensing systems would assist risk management of these chemicals. Here, we characterise the ethylene regulatory system in Mycobacterium strain NBB4 and use these genetic parts to create a biosensor. The regulatory genes etnR1 and etnR2 and cognate promoter Petn were combined with a fluorescent reporter gene (fuGFP) in a Mycobacterium shuttle vector to create plasmid pUS301‐EtnR12P. Cultures of M. smegmatis mc2‐155(pUS301‐EtnR12P) gave a fluorescent signal in response to ethylene oxide with a detection limit of 0.2 μM (9 ppb). By combining the epoxide biosensor cells with another culture expressing the ethylene monooxygenase, the system was converted into an ethylene biosensor. The co‐culture was capable of detecting ethylene emission from banana fruit. These are the first examples of whole‐cell biosensors for epoxides or aliphatic alkenes. This work also resolves long‐standing questions concerning the regulation of ethylene catabolism in bacteria.
- Subjects
BIOSENSORS; ETHYLENE oxide; REGULATOR genes; REPORTER genes; FRUIT ripening; CHEMICAL industry; BANANAS; DETECTION limit
- Publication
Microbial Biotechnology, 2024, Vol 17, Issue 6, p1
- ISSN
1751-7907
- Publication type
Article
- DOI
10.1111/1751-7915.14511