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- Title
PEP-1-SOD1 fusion proteins block cardiac myofibroblast activation and angiotensin II-induced collagen production.
- Authors
Li-Guo Tan; Jun-Hui Xiao; Dan-Li Yu; Lei Zhang; Fei Zheng; Ling-Yun Guo; Jian-Ye Yang; Jun-ming Tang; Shi-You Chen; Jia-Ning Wang; Tan, Li-Guo; Xiao, Jun-Hui; Yu, Dan-Li; Zhang, Lei; Zheng, Fei; Guo, Ling-Yun; Yang, Jian-Ye; Tang, Jun-Ming; Chen, Shi-You; Wang, Jia-Ning
- Abstract
<bold>Background: </bold>Oxidative stress is closely associated with cardiac fibrosis. However, the effect of copper, zinc-superoxide dismutase (SOD1) as a therapeutic agent is limited due to the insufficient transduction. This study was aimed to investigate the effect of PEP-1-SOD1 fusion protein on angiotensin II (ANG II)-induced collagen metabolism in rat cardiac myofibroblasts (MCFs). <bold>Methods: </bold>MCFs were pretreated with SOD1 or PEP-1-SOD1 fusion protein for 2 h followed by incubation with ANG II for 24 h. Cell proliferation was measured by Cell Counting Kit-8. Superoxide anion productions were detected by both fluorescent microscopy and Flow Cytometry. MMP-1 and TIMP-1 were determined by ELISA. Intracellular MDA content and SOD activity were examined by commercial assay kits. Protein expression was analyzed by western blotting. <bold>Results: </bold>PEP-1-SOD1 fusion protein efficiently transduced into MCF, scavenged intracellular O2 (-), decreased intracellular MDA content, increased SOD activity, suppressed ANG II-induced proliferation, reduced expression of TGF-β1, α-SMA, collagen type I and III, restored MMP-1 secretion, and attenuated TIMP-1 secretion. <bold>Conclusion: </bold>PEP-1-SOD1 suppressed MCF proliferation and differentiation and reduced production of collagen type I and III. Therefore, PEP-1-SOD1 fusion protein may be a potential novel therapeutic agent for cardiac fibrosis.
- Subjects
PEPTIDES; SUPEROXIDE dismutase; CHIMERIC proteins; HEART fibrosis; MYOFIBROBLASTS; ANGIOTENSIN II; COLLAGEN; OXIDATIVE stress; THERAPEUTICS; AMINES; ANIMAL experimentation; CELL physiology; FIBROBLASTS; PEROXIDES; PROTEINS; PROTEOLYTIC enzymes; RATS; RESEARCH funding; MALONDIALDEHYDE; METABOLISM
- Publication
BMC Cardiovascular Disorders, 2015, Vol 15, Issue 1, p1
- ISSN
1471-2261
- Publication type
journal article
- DOI
10.1186/s12872-015-0103-4