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- Title
A Novel 66-kDa Stress Protein, p66, Associated with the Process of Cyst Formation of Physarum polycephalum Is a Physarum Homologue of a Yeast Actin-Interacting Protein, AIP11.
- Authors
Matsumoto, Seiji; Ogawa, Mari; Kasakura, Tomoko; Shimada, Yukiko; Mitsui, Makoto; Maruya, Mikako; Isohata, Michiko; Yahara, Ichiro; Murakami-Murofushi, Kimiko
- Abstract
When exposed to various stresses including heat shock, myxoamoebae, growing haploid cells of Physarum polycephalum, show marked morphological changes and consequently become disk-shaped microcysts. We have found that p66 is induced exclusively in the course of microcyst formation and has an actin-binding activity. In this study, we purified p66 to homogeneity and isolated a p66 cDNA. The deduced protein sequence contained 601 amino acids and showed 31% identity to a yeast actin-interacting protein, AIP1. Northern blot analysis revealed that the amount of p66 mRNA was significantly increased by heat shock in myxoamoebae but not in plasmodia. Thus, p66 seems to be a developmentally-expressed stress protein which regulates the rearrangement of actin organization during microcyst formation in P. polycephalum.
- Subjects
PROTEINS; PHYSARUM polycephalum; AMINO acid sequence; HEAT shock proteins; LEAVENING agents
- Publication
Journal of Biochemistry, 1998, Vol 124, Issue 2, p326
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a022115