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- Title
PRELIMINARY STRUCTURAL CHARACTERIZATION, ANTIOXIDANT AND ANTITUMOR ACTIVITIES OF CHINESE CHESTNUT (CASTANEA MOLLISSIMA BLUME) POLYSACCHARIDES.
- Authors
Tong-Kun Wang; Bin Du; Yue-Dong Yang; Xue Liang; Xiao-Hong Wang; Yan Zhou
- Abstract
A new polysaccharide was extracted from Chinese chestnut (Castanea mollissima Blume) by pressurized liquid extraction (PLE) and further successive purification through DEAE-cellulose 52 and Sephadex G-200 columns. The results showed a single and symmetrical sharp peak, which suggested that purified polysaccharide was homogeneous. The molecular weight was calculated to be 3.28 × 105 Da. Monosaccharide analysis revealed that the polysaccharide was composed of rhamnose, ribose, arabinose, xylose, mannose, glucose, and D-galactose in the molar ratio of 1.42%, 13.12%, 22.68%, 1.57%, 9.39%, 21.58%, 30.24%, respectively. There was a dose-dependent relationship with measures of antioxidant activities, viz. capabilities to scavenge free radical, superoxide anion free radical, hydrogen peroxides and reducibility. The antitumor activity of the polysaccharide was tested in a cell culture model. The polysaccharide caused a significant dosedependent inhibition on the multiplication of cancer cells.
- Subjects
ANTIOXIDANTS; ANTINEOPLASTIC agents; CHINESE chestnut; POLYSACCHARIDES; MOLECULAR weights; CELL culture
- Publication
Current Topics in Nutraceutical Research, 2015, Vol 13, Issue 4, p241
- ISSN
1540-7535
- Publication type
Article