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- Title
Altered Metabolic Profile of Triglyceride-Rich Lipoproteins in Gut-Lymph of Rodent Models of Sepsis and Gut Ischemia-Reperfusion Injury.
- Authors
Hong, Jiwon; Nachkebia, Shorena; Tun, Soe Min; Petzer, Amorita; Windsor, John A.; Hickey, Anthony J.; Phillips, Anthony R.
- Abstract
<bold>Background: </bold>Triglyceride-rich lipoproteins are important in dietary lipid absorption and subsequent energy distribution in the body. Their importance in the gut-lymph may have been overlooked in sepsis, the most common cause of critical illness, and in gut ischemia-reperfusion injury, a common feature of many critical illnesses.<bold>Aims: </bold>We aimed to undertake an exploratory study of triglyceride-rich lipoprotein fractions in gut-lymph using untargeted metabolic profiling to identify altered metabolites in sepsis or gut ischemia-reperfusion.<bold>Methods: </bold>The gut-lymph was collected from rodent sham, sepsis, and gut ischemia-reperfusion models. The triglyceride-rich lipoprotein-enriched fractions isolated from the gut-lymph were subjected to a dual metabolomics analysis approach: non-polar metabolite analysis by ultra-high performance liquid chromatography-mass spectrometry and polar metabolite analysis by gas chromatography-mass spectrometry.<bold>Results: </bold>The metabolite analysis of gut-lymph triglyceride-rich lipoprotein fractions revealed a significant increase (FDR-adjusted P value < 0.05) in myo-inositol in the sepsis group and monoacylglycerols [(18:1) and (18:2)] in gut ischemia-reperfusion. There were no significantly increased specific metabolites in the lipoprotein-enriched fractions of both sepsis and gut ischemia-reperfusion. In contrast, there was a widespread decrease in multiple lipid species in sepsis (35 out of 190; adjusted P < 0.05), but not in the gut ischemia-reperfusion.<bold>Conclusions: </bold>Increased levels of myo-inositol and monoacylglycerols, and decreased multiple lipid species in the gut-lymph triglyceride-rich lipoprotein fraction could be candidates for new biomarkers and/or involved in the progression of sepsis and gut ischemia-reperfusion pathobiology.
- Subjects
TRIGLYCERIDES; LIPOPROTEINS; SEPSIS; REPERFUSION injury; RODENTS as carriers of disease; ANIMAL experimentation; BIOCHEMISTRY; BIOLOGICAL models; CHYLE; GLYCERIDES; INOSITOL; LIQUID chromatography; MASS spectrometry; RATS
- Publication
Digestive Diseases & Sciences, 2018, Vol 63, Issue 12, p3317
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-018-5270-6