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- Title
Direct induction of human B-cell differentiation by recombinant interleukin-2.
- Authors
Romagnani, S.; Del Prete, G.; Giudizi, Maria O.; Biagiotti, Roberta; Almerigogna, F.; Tiri, A.; Alessi, Anna; Mazzetti, M.; Ricci, M.
- Abstract
Recombinant interleukin-2 (rIL-2) induced highly purified human tonsillar B cells to differentiate into immunoglobulin (Ig)-producing cells in vitro. The B-cell response was not due to rIL-2- contaminating substances, but reflected the activity of IL-2 itself, since it was inhibited by addition to the cultures of anti-TAC monoclonal antibody. The rIL-2-induced B-cell response was apparently not mediated by factors released by residual T cells present in B-cell suspensions at undetectable levels, since supernatants (SN) from unstimulated autologous T cells cultured at concentrations even much higher than those possibly contaminating B-cell suspensions did not induce any detectable Ig production. In addition, the Ig production by B cells cultured with SN prepared from high numbers of autologous T cells stimulated with rIL-2, as well as from allo-activated or mitogen-stimulated T cells, was of the same magnitude as the Ig production resulting from direct addition of rIL-2 concentrations comparable with those present in the supernatants. After centrifugation on Percoll density gradients, most of the tonsillar B cells responsive to rIL-2 were recovered in the lower density cell fraction containing a number of larger activated B cells. Moreover, B-cell enriched suspensions from peripheral blood (PB) (which usually contains a lower number of in vivo activated B cells than tonsil) showed poor or no response to rIL-2 alone, but displayed significant Ig production when rIL-2 was added to the cultures in the presence of Staphylococcus aureus Cowan I (SAC) bacteria. Taken together, these data indicate that IL-2 can directly induce differentiation into Ig-producing cells of in vivo or in vitro activated human B cells.
- Subjects
INTERLEUKINS; B cell differentiation; INTERLEUKIN-2; B cells; CELL differentiation; CELL culture
- Publication
Immunology, 1986, Vol 58, Issue 1, p31
- ISSN
0019-2805
- Publication type
Article