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- Title
Mass spectrometry-based proteomic characterization of the middleaged mouse brain for animal model research of neuromuscular diseases.
- Authors
Dowling, Paul; Zweyer, Margit; Sabir, Hemmen; Henry, Michael; Meleady, Paula; Swandulla, Dieter; Ohlendieck, Kay
- Abstract
Neuromuscular diseases with primary muscle wasting symptoms may also display multisystemic changes in the body and exhibit secondary pathophysiological alterations in various non-muscle tissues. In some cases, this includes proteome-wide alterations and/or adaptations in the central nervous system. Thus, in order to provide an improved bioanalytical basis for the comprehensive evaluation of animal models that are routinely used in muscle research, this report describes the mass spectrometry-based proteomic characterization of the mouse brain. Crude tissue extracts were examined by bottom-up proteomics and detected 4558 distinct protein species. The detailed analysis of the brain proteome revealed the presence of abundant cellular proteoforms in the neuronal cytoskeleton, as well as various brain region enriched proteins, including markers of the cerebral cortex, cerebellum, hippocampus and the olfactory bulb. Neuroproteomic markers of specific cell types in the brain were identified in association with various types of neurons and glia cells. Markers of subcellular structures were established for the plasmalemma, nucleus, endoplasmic reticulum, mitochondria and other crucial organelles, as well as synaptic components that are involved in presynaptic vesicle docking, neurotransmitter release and synapse remodelling.
- Subjects
NEUROMUSCULAR diseases; NEUROMUSCULAR transmission; ANIMAL models in research; NEURAL transmission; CENTRAL nervous system; PROTEOMICS; CEREBRAL cortex
- Publication
European Journal of Translational Myology, 2023, Vol 33, Issue 3, p1
- ISSN
2037-7452
- Publication type
Article
- DOI
10.4081/ejtm.2023.11553