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- Title
AN INVESTIGATION ON IN VITRO CULTURE OF SUGAR BEET (BETA VULGARIS L.) USING LIGHT AND FLUORESCENT MICROSCOPY.
- Authors
Whiteside, Mandy; Villanueva, Esther; Caraway, Edward; Ghosh, Nabarun; Smith, Don W.
- Abstract
Sugar beet (Beta vulgaris L.) is a member of the fam̀ily Amaranthaceae, subfamily Chenopodiaceae. Sugar beet roots contain 15-20% sucrose representing a major source for the sweetener industry. Rhizomania, the most devastating disease caused by BSBMV (Beet Soil Borne Mosaic Virus) and BNYVV (Beet Necrotic Yellow Vein Virus) resulted in vast decline of production in United States in the last decade. We established in vitro cultures of sugar beet for the regeneration of improved varieties and to study the pathogenesis from systemically infected tissue in culture. We excised the hypocotyl and cotyledon explants from the seedlings of Beta-1395 germinated on ½ MS medium and implanted them into modified MS medium. After 21 days culture, callusing was observed from the cut ends of the explants. Development of shoot was achieved by the addition of various growth factors and coconut milk (5% v/v) to MS medium. Rhizogenesis was obtained using 2 mg/L of IAA to MS medium. After three weeks of transfer, the formation of roots at the bottom of the regenerated shootlets was recorded. Using callus we established cell suspension cultures to obtain protoplasts for further experimentation. The morphogenesis process was studied using light and fluorescent microscopy. Staining the cultured cells with vital stain Evan's Blue helped us screen the regenerative cells from suspension culture. We observed the torpedo shaped embryonic initial that exhibited characteristic fluorescence with FITC filter (Fig. 1).
- Subjects
UNITED States; SUGAR beets; BEET diseases &; pests; MICROSCOPY; PLANT growing media; PLANT roots; CELL culture
- Publication
Texas Journal of Microscopy, 2007, Vol 38, Issue 1, p11
- ISSN
1554-0820
- Publication type
Article