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- Title
Kinetic and stoichiometric parameters in the fed-batch bioreactor production of poly(3-hydroxybutyrate) by Bacillus megaterium using different carbon sources.
- Authors
De Melo, Rafaela Nery; de Souza Hassemer do, Guilherme; Nascimento, Lucas Henrique; Colet, Rosicler; Steffens, Clarice; Junges, Alexander; Valduga, Eunice
- Abstract
This study investigates the effects of different strategies on poly(3-hydroxybutyrate)-P(3HB) production in a fed-batch bioreactor by Bacillus megaterium using candy industry effluent (CIE), sucrose, and rice parboiled water (RPW) as carbon sources. In biosynthesis, kinetic and stoichiometric parameters of substrate conversion into products and/or cells, productivity, instantaneous, and specific conversion rates were evaluated. The maximum concentration of P(3HB) was 4.00 g.L−1 (77% of the total dry mass) in 42 h of cultivation in minimal medium/RPW added with a carbon source based on CIE, demonstrating that the fed-batch provided an increase of approximately 22% in the polymer concentration and 32% in the overall productivity in relation to medium based on commercial sucrose. Fed-batch cultivation also had the advantage of avoiding the extra time required for inoculum preparation and sterilization of the bioreactor during the batch, which thereby increased the overall industrial importance of the process. Effluents from the candy, confectionery, and/or rice parboiling industries can be used as alternative substrates for P(3HB) production at a low cost.
- Subjects
BACILLUS megaterium; PARBOILED rice; 3-Hydroxybutyric acid; CANDY industry; SUCROSE; RICE industry
- Publication
Bioprocess & Biosystems Engineering, 2023, Vol 46, Issue 12, p1791
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-023-02935-9