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- Title
Heavy-ion beam mutagenesis of the ectomycorrhizal agaricomycete Tricholoma matsutake that produces the prized mushroom 'matsutake' in conifer forests.
- Authors
Murata, Hitoshi; Abe, Tomoko; Ichida, Hiroyuki; Hayashi, Yoriko; Yamanaka, Takashi; Shimokawa, Tomoko; Tahara, Ko
- Abstract
Tricholoma matsutake is an ectomycorrhizal agaricomycete that produces the prized mushroom 'matsutake' in Pinaceae forests. Currently, there are no available cultivars or cultivation methods that produce fruiting bodies. Heavy-ion beams, which induce mutations through double-stranded DNA breaks, have been used widely for plant breeding. In the present study, we examined whether heavy-ion beams could be useful in isolating T. matsutake mutants. An argon-ion beam gave a suitable lethality curve in relation to irradiation doses, accelerating killing at 100-150 Gy. Argon-ion beam irradiation of the agar plate cultures yielded several transient mutants whose colony morphologies differed from that of the wild-type strain at the first screening, but which did not persist following culture transfer. It also generated a mutant whose phenotype remained stable after repeated culture transfers. The stable pleiotropic mutant not only exhibited a different colony morphology to the wild type, but also showed increased degradation of dye-linked water-insoluble amylose and cellulose substrates. Thus, heavy-ion beams may be useful for isolating mutants of T. matsutake, although precautions may be required to maintain the mutants, without phenotypic reversion, during repetitive culture of their mycelia.
- Subjects
TRICHOLOMA matsutake; GERMINATION; BIODIVERSITY; CYANOBACTERIA; DNA repair
- Publication
Mycorrhiza, 2018, Vol 28, Issue 2, p171
- ISSN
0940-6360
- Publication type
Article
- DOI
10.1007/s00572-017-0810-z