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- Title
Bioconversion of (+)-valencene in submerged cultures of the ascomycete Chaetomium globosum.
- Authors
Kaspera, Rüdiger; Krings, Ulrich; Nanzad, Tsevegsuren; Berger, Ralf G.
- Abstract
Submerged cultures of the ascomycete Chaetomium globosum oxidised the exogenous sesquiterpene (+)-valencene to nootkatone via the stereoselective generation of α-nootkatol. Inhibition experiments suggested that the first introduction of oxygen, the rate-limiting step of the bioconversion, may have been catalysed by a cytochrome-P450-monooxygenase. However, nootkatone was not the final metabolite: further flavour-active and inactive, non-volatile oxidation products were identified. (+)-Valencene and the flavour-active mono-oxyfunctionalised transformation products, α-nootkatol, nootkatone, and valencene-11,12-epoxide accumulated preferably inside the fungal cells. Di- and poly-oxygenated products, such as nootkatone-11,12-epoxide, were found solely in the culture medium, indicating an active transport of these metabolites into the extracellular compartment during (+)-valencene detoxification. These metabolic properties may have contributed to the high tolerance of the fungus towards the exogenous hydrocarbon.
- Subjects
ASCOMYCETES; CHAETOMIUM globosum; SESQUITERPENES; FUNGAL cytology; BIOTECHNOLOGY
- Publication
Applied Microbiology & Biotechnology, 2005, Vol 67, Issue 4, p477
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-004-1794-0