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- Title
Protein kinase C-δ is involved in induction of NOX1 gene expression by aldosterone in rat vascular smooth muscle cells.
- Authors
Haiyan Wei; Xuhua Mi; Ling Ji; Lichuan Yang; Qingjie Xia; Yuquan Wei; Miyamori, Isamu; ChunYuan Fan
- Abstract
In this study, we focused on the relationship between aldosterone and NOX1 expression in vascular smooth muscle cells (VSMCs). For the first time, with the use of specific inhibitors of protein kinase C (PKC), we report that PKCδ mediates upregulation of NOX1 induced by 10 nM aldosterone in cultured VSMCs. Participation of PKC in the mediation of NOX1 regulation was further confirmed by the effect of diacylglycerol, a PKC agonist, on the NOX1 RNA in A7r5 cells with Northern blot analysis. To establish cause and effect, we next silenced the PKCδ gene partly by RNA interference and found knockdown of PKCδ gene attenuated aldosterone-induced NOX1 expression, generation of superoxide, as well as protein synthesis in VSMCs. Taken together, these data indicated PKCδ might mediate aldosterone-dependent NOX1 upregulation in VSMCs. In addition, we showed that the cascade from aldosterone to PKCδ activation had the participation of the mineralocorticoid receptor.
- Subjects
PROTEIN kinase C; GENE expression; ALDOSTERONE; LABORATORY rats; SMOOTH muscle
- Publication
Biochemistry (00062979), 2010, Vol 75, Issue 3, p304
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297910030065