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- Title
Trehalose accumulation from cassava starch and release by a highly thermosensitive and permeable mutant of Saccharomycopsis fibuligera.
- Authors
Wang, Dong-Sheng; Zhao, Shou-Feng; Zhao, Ming-Xin; Li, Jing; Chi, Zhen-Ming
- Abstract
Highly thermosensitive and permeable mutants are the mutants from which intracellular contents can be released when they are incubated both in low osmolarity water and at non-permissive temperature (usually 37°C). After mutagenesis by using nitrosoguanidine, a highly thermosensitive and permeable mutant named A11-b was obtained from Saccharomycopsis fibuligera A11-12, a trehalose overproducer in which the acid protease gene has been disrupted. Of the total trehalose, 73.8% was released from the mutant cells suspended in distilled water after they had been treated at 37°C overnight. However, only 10.0% of the total trehalose was released from the cells of S. fibuligera A11-12 treated under the same conditions. The cell volume of the mutant cells suspended in distilled water and treated at 37°C overnight was much bigger than that of S. fibuligera A11-12 treated under the same conditions. The cell growth and trehalose accumulation of the mutant were almost the same as those of S. fibuligera A11-12 during the cultivation at the flask level and in a 5-l fermentor. Both could accumulate around 28.0% (w/w) trehalose from cassava starch. After purification, the trehalose crystal from the aqueous extract of the mutant was obtained.
- Subjects
CASSAVA; YEAST; MUTAGENESIS; BIOACCUMULATION; STARCH; OSMOLAR concentration; EXTRACTION (Chemistry)
- Publication
Journal of Industrial Microbiology & Biotechnology, 2011, Vol 38, Issue 9, p1545
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-011-0943-6