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- Title
Enhanced assembly of bacteriophage T7 produced in cell-free reactions under simulated microgravity.
- Authors
Lehr, François-Xavier; Pavletić, Bruno; Glatter, Timo; Heimerl, Thomas; Moeller, Ralf; Niederholtmeyer, Henrike
- Abstract
On-demand biomanufacturing has the potential to improve healthcare and self-sufficiency during space missions. Cell-free transcription and translation reactions combined with DNA blueprints can produce promising therapeutics like bacteriophages and virus-like particles. However, how space conditions affect the synthesis and self-assembly of such complex multi-protein structures is unknown. Here, we characterize the cell-free production of infectious bacteriophage T7 virions under simulated microgravity. Rotation in a 2D-clinostat increased the number of infectious particles compared to static controls. Quantitative analyses by mass spectrometry, immuno-dot-blot and real-time PCR showed no significant differences in protein and DNA contents, suggesting enhanced self-assembly of T7 phages in simulated microgravity. While the effects of genuine space conditions on the cell-free synthesis and assembly of bacteriophages remain to be investigated, our findings support the vision of a cell-free synthesis-enabled "astropharmacy".
- Subjects
REDUCED gravity environments; BACTERIOPHAGES; VIRUS-like particles; GREEN business
- Publication
NPJ Microgravity, 2024, Vol 10, Issue 1, p1
- ISSN
2373-8065
- Publication type
Article
- DOI
10.1038/s41526-024-00378-4