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- Title
Effects of a sucrose-enriched diet on the pattern of gene expression, contraction and Ca<sup>2+</sup> transport in Goto-Kakizaki type 2 diabetic rat heart.
- Authors
Gaber, E. M.; Jayaprakash, P.; Qureshi, M. A.; Parekh, K.; Oz, M.; Adrian, T. E.; Howarth, F. C.
- Abstract
New Findings What is the central question of this study? Poor diet is a risk factor for development of type 2 diabetes mellitus and its associated complications. In this study, the effects of sucrose-enriched diet on the pattern of gene expression, contraction and Ca2+ transport in type 2 diabetic heart are explored., What is the main finding and its importance? The altered pattern of gene expression in type 2 diabetic hearts was further altered in diabetic and control rats that received a sucrose-enriched diet, and these alterations were associated with changes in ventricular myocyte shortening and Ca2+ transport., There has been a spectacular rise in the global prevalence of type 2 diabetes mellitus (T2DM), and cardiovascular disease is the major cause of morbidity and mortality in diabetic patients. A variety of diastolic and systolic dysfunctions have been demonstrated in type 2 diabetic heart. The consumption of sugar-sweetened beverages has been linked to rising rates of obesity, which in turn is a risk factor for development of T2DM. In this study, the effects of a sucrose-enriched diet on the pattern of gene expression, contraction and Ca2+ transport in the Goto-Kakizaki T2DM rat heart were investigated. Genes encoding cardiac muscle proteins ( Myh7, Mybpc3, Myl1, Myl3 and Mylpf), intercellular proteins ( Gja4), cell membrane transport ( Atp1b1), calcium channels ( Cacna1c, Cacna1g and Cacnb1) and potassium channels ( Kcnj11) were upregulated and genes encoding potassium channels ( Kcnb1) were downregulated in GK compared with control rats. Genes encoding cardiac muscle proteins ( Myh6, Mybpc3 and Tnn2), intercellular proteins ( Gja1 and Gja4), intracellular Ca2+ transport ( Atp2a1 and Ryr2), cell membrane transport ( Atp1a2 and Atp1b1) and potassium channel proteins ( Kcnj2 and Kcnj8) were upregulated and genes encoding cardiac muscle proteins ( Myh7) were downregulated in control rats fed sucrose compared with control rats. Genes encoding cardiac muscle proteins ( Myh7) and potassium channel proteins ( Kcnj11) were downregulated in control and GK rats fed sucrose compared with control and GK rats, respectively. The amplitude of shortening was reduced in myocytes from the control-sucrose group compared with control rats and in the GK-sucrose group compared with GK rats. The amplitude of the Ca2+ transient was increased in myocytes from control-sucrose compared with control rats and decreased in GK-sucrose compared with GK rats. Subtle alterations in the pattern of expression of genes encoding a variety of cardiac muscle proteins are associated with changes in shortening and intracellular Ca2+ transport in ventricular myocytes from GK T2DM and control rats fed a sucrose-enriched diet.
- Subjects
SUCROSE; GENE expression; CARDIAC contraction; CALCIUM-binding proteins; TYPE 2 diabetes; PEOPLE with diabetes; MUSCLE proteins
- Publication
Experimental Physiology, 2014, Vol 99, Issue 6, p881
- ISSN
0958-0670
- Publication type
Article
- DOI
10.1113/expphysiol.2013.077594