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- Title
Purification and Characterization of an Isolate of Apple Mosaic Virus from Rose in the USA.
- Authors
Wong, Sek-man; Horst, R. Kenneth
- Abstract
An isolate of apple mosaic virus, designated ApMV-J, was isolated from rose cv. Sweet Surrender in New York State, USA. Mechanical transmission of ApMV-J from rose to cucumber was facilitated by preparing inocula with 0.03 M sodium phosphate buffer containing 0.02 M 2-mercaptoethanol and 1% polyvinylpyrolidone. <em>Catharanthus roseus</em> cv. Little Linda was a good virus maintenance host and <em>Cucumis sativus</em> cv. Lemon a good propagation host. Contaminating host proteins were substantially reduced by including polyethylene glycol in the purification protocol. Purified virus had high A260/280nm values and contained virus particles of c 25-26 nm in diameter; most particles were isometric but a few were bacilliform. The M. Wts. of the four nucleic acids of ApMV-J, determined in non-denaturing and denaturing agarose gels, were estimated at 1.16, 0.98, 0.68 and 0.35 × 106 and 1.0, 0.91 0.66 and 0.35 × 106, respectively. The viral coat protein M.Wt. was estimated to be 27 kDa.
- Subjects
VIRUSES; CATHARANTHUS; POLYETHYLENE glycol; POLYOLS; ETHYLENE glycol; NUCLEIC acids
- Publication
Journal of Phytopathology, 1993, Vol 139, Issue 1, p33
- ISSN
0931-1785
- Publication type
Article
- DOI
10.1111/j.1439-0434.1993.tb01399.x