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- Title
Cyclooxygenase expression in splanchnic hyposensitivity to glypressin of bleeding portal hypertensive rats.
- Authors
Huang, H.-C.; Wang, S.-S.; Chen, Y.-C.; Lee, F.-Y.; Chang, F.-Y.; Lin, H.-C.; Hou, M.-C.; Chan, C.-C.; Chen, C.-T.; Wu, S.-L.; Lee, S.-D.
- Abstract
Abstract Background Prostacyclin mediates, at least partly, the splanchnic vascular hyporesponsiveness to glypressin in bleeding portal hypertensive rats. This study investigated the relative contribution of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) in the splanchnic hyposensitivity to glypressin in rats with portal hypertension induced by partial portal vein ligation (PVL). Methods Fourteen days after the operation, the rats were divided into without- and with-bleeding groups. Three series of PVL rats were used to investigate (i) the haemodynamic effects of glypressin (0·07 mg kg-1 intravenously), (ii) COX-1/COX-2 mRNA expression over abdominal aorta and superior mesenteric artery and (iii) plasma levels of 6-keto-prostaglandin-F1α . In rats with a hypotensive haemorrhage, 4·5 mL of blood was withdrawn and 50% of the withdrawn blood was re-infused before blood and vessel sampling or the administration of glypressin. Results Splanchnic hyposensitivity to glypressin was demonstrated in the haemorrhage-transfused PVL rats with enhanced COX-1 expression of superior mesenteric artery and increased plasma levels of 6-keto-prostaglandin-F1α . There were no differences in the COX-2 expression of superior mesenteric artery and COX-1 and COX-2 expressions of abdominal aorta between without- and with-bleeding groups. Conclusion In portal hypertensive rats with acute haemorrhage, COX-1 over-expression in the superior mesenteric artery plays a role in mediating the splanchnic hyposensitivity to glypressin.
- Subjects
CYCLOOXYGENASES; HYPERTENSION; RATS
- Publication
European Journal of Clinical Investigation, 2003, Vol 33, Issue 6, p505
- ISSN
0014-2972
- Publication type
Article
- DOI
10.1046/j.1365-2362.2003.01162.x