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- Title
Effect of Silver Diamine Fluoride Treatment on Microbial Profiles of Plaque Biofilms from Root/Cervical Caries Lesions.
- Authors
Mitwalli, Heba; Mourao, Marcio D.A.; Dennison, Joseph; Yaman, Peter; Paster, Bruce J.; Fontana, Margherita; Mourao, Marcio D A; Paster, Bruce J
- Abstract
<bold>Purpose: </bold>To assess the effect of silver diamine fluoride (SDF) on microbial profiles present in plaque from root/cervical carious lesions, and its association with caries lesion arrest.<bold>Materials and Methods: </bold>Twenty patients with at least one soft cavitated root/cervical carious lesion were included. One lesion/patient was randomly selected and treated with 38% SDF. Supragingival plaque samples were harvested at preintervention and 1 month postintervention. Using an MiSeq platform, 16S rDNA sequencing of the V3-V4 regions was used to determine bacterial profiles. Clinical evaluation of lesion hardness was used to evaluate arrest. t tests, principal component analysis (PCA), multidimensional scaling (MDS), and generalized linear models (GLMs) tests were used for statistical comparisons.<bold>Results: </bold>From a total of 40 plaque samples, 468 probe targets were observed. Although 60% of lesions became hard postintervention, PCA and MDS tests showed no distinct pre- and postintervention groups. In addition, pre- and postintervention differences in diversity (Shannon index) of microbial profiles between patients with and without lesion arrest were not statistically different. A likelihood ratio test for pre- versus postintervention differences within patients, i.e., adjusting for differences between patients using negative binomial GLMs, showed 17 bacterial taxa with significant differences (FDR <0.05).<bold>Conclusion: </bold>Although 60% of lesions hardened after SDF treatment, this was not directly due to either overall statistically significant differences in microbial profiles or differences in microbial diversity. Nevertheless, there was a trend with some acid-producing species in that their relative abundance was reduced postintervention. The negative binomial GLMs showed 17 bacterial taxa that were significantly different after SDF treatment.
- Subjects
FALSE discovery rate; DENTAL caries; DENTAL fluoride treatment; BACTERIA classification; RESEARCH; SILVER compounds; DENTAL plaque; FLUORIDE varnishes; RESEARCH methodology; BIOFILMS; EVALUATION research; MULTIDIMENSIONAL Health Locus of Control scales; COMPARATIVE studies; CARIOSTATIC agents; QUATERNARY ammonium compounds; BACTERIA; PHARMACODYNAMICS
- Publication
Caries Research, 2019, Vol 53, Issue 5, p555
- ISSN
0008-6568
- Publication type
journal article
- DOI
10.1159/000499578