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- Title
Ca<sup>2+</sup> Signaling Tools Acquired from Prostasomes Are Required for Progesterone-Induced Sperm Motility.
- Authors
Kwang-Hyun Park; Byung-Ju Kim; John Kang; Tae-Sik Nam; Jung Min Lim; Hyoung Tae Kim; Jong Kwan Park; Young Gon Kim; Soo-Wan Chae; Uh-Hyun Kim
- Abstract
Progesterone-induced calcium ion (Ca2+) signals in the neck region of sperm play a pivotal role in promoting sperm motility. Here, we show that a long-lasting Ca2+ signal required for sperm motility in response to progesterone depends on their pH-dependent fusion with prostasomes, which are small vesicles secreted by the prostate. We found that prostasome fusion led to the transfer of progesterone receptors, cyclic adenosine diphosphoribose (cADPR)-synthesizing enzymes, ryanodine receptors (RyRs), and other Ca2+ signaling tools from prostasomes to the sperm neck. Progesterone-induced sperm motility relied on cADPR-mediated Ca2+ mobilization through RyR located on acidic Ca2+ stores, followed by Ca2+ entry through store-operated channels. Treatment of prostasome-fused sperm with a cADPR antagonist or fusion with prostasomes in which type 2 RyR was depleted resulted in low fertilization rates, reduced sperm motility, or both. Thus, we conclude that sperm motility depends on the acquisition of Ca2+ signaling tools from prostasomes.
- Publication
Science Signaling, 2011, Vol 4, Issue 173, p1
- ISSN
1945-0877
- Publication type
Article
- DOI
10.1126/scisignal.2001595