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- Title
The Diamagnetic Susceptibility of the Tubulin Dimer.
- Authors
Bras, Wim; Torbet, James; Diakun, Gregory P.; Rikken, Geert L. J. A.; Diaz, J. Fernando
- Abstract
An approximate value of the diamagnetic anisotropy of the tubulin dimer, Δχdimer, has been determined assuming axial symmetry and that only the α-helices and β-sheets contribute to the anisotropy. Two approaches have been utilized: (a) using the value for the Δχ α for an α-helical peptide bond given by Pauling (1979) and (b) using the previously determined anisotropy of fibrinogen as a calibration standard. The Δχdimer ≈ 4 × 10-27 JT-2 obtained from these measurements are similar to within 20%. Although Cotton-Mouton measurements alone cannot be used to estimate Δχ directly, the value we measured, CMdimer = (1.41 ± 0.03) × 10-8 T-2 cm² mg-1, is consistent with the above estimate for Δχdimer. Themethod utilized for the determination of the tubulin dimer diamagnetic susceptibility is applicable to other proteins and macromolecular assemblies as well.
- Subjects
DIAMAGNETIC materials; MAGNETIC susceptibility; TUBULINS; DIMERS; MAGNETIC anisotropy; PEPTIDE bonds; COTTON-Mouton effect
- Publication
Journal of Biophysics, 2014, p1
- ISSN
1687-8000
- Publication type
Article
- DOI
10.1155/2014/985082