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- Title
Development of a High-Performance Liquid Chromatographic Method for Determination of Letrozole in Wistar Rat Serum and its Application in Pharmacokinetic Studies.
- Authors
ACHARJYA, Sasmita Kumari; BHATTAMISRA, Subrat Kumar; MUDDANA, Bhanoji Rao E.; BERA, Ravikumar V. V.; PANDA, Pinakini; PANDA, Bibhu Prasad; MISHRA, Gitanjali
- Abstract
A fast, sensitive, and specific reversed-phase high-performance liquid chroma-tographic (RP-HPLC) method for the determination of letrozole in Wistar rat serum was developed. In this method, liquid-liquid extraction of letrozole was achieved using diethyl ether as the extracting solvent. The analysis was carried out on a reversed-phase C18 (250 mm × 4.6 mm, 5 μm) column with an isocratic mobile phase of methanol-water (70:30,v/v), at a flow rate of 1.0 mL min-1. Detection was carried out at 239 nm with a UV-visible spectrophoto-metric detector. The method was shown to be selective and linear over the concentration range of 0.15-100 μg mL-1. The intra-day and inter-day precision studies showed good reproducibility with coefficients of variation less than 11% for the analyte. The relative errors of intra- and inter-day accuracy were within -11.52 to -2.26%. The limit of quantification was evaluated to be 0.15 μg mL-1. The method was successfully applied for the pharmacokinetic study of letrozole after oral administration of 10 mg kg-1 of letrozole in six healthy Wistar rats.
- Subjects
LIQUID chromatography; LETROZOLE; ANTINEOPLASTIC agents; PHARMACOKINETICS; LABORATORY rats; ETHERS
- Publication
Scientia Pharmaceutica, 2012, Vol 80, Issue 4, p941
- ISSN
0036-8709
- Publication type
Article
- DOI
10.3797/scipharm.1206-06