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- Title
Aptamer based fluorometric β-lactoglobulin assay based on the use of magnetic nanoparticles and carbon dots.
- Authors
Menglan Shi; Yao Cen; Muhammad Sohail; Guanhong Xu; Fangdi Wei; Yunsu Ma; Xiaoman Xu; Yujie Ma; Yueyue Song; Qin Hu
- Abstract
The authors describe a fluorometric aptamer based assay for detecting β-lactoglobulin by using carbon dots (C-dots) as a signal indicator. The aptamer was immoblized on magnetite (Fe3O4) nanoparticles (MNPs), and the C-dots served as a label for the complementary oligonucleotide (cDNA). The assay is based on the hybridization that takes place between aptamer and cDNA. In the presence of β-lactoglobulin (β-LG), the aptamer preferentially binds to β-LG, and this leads to a partial release of the C-dotscDNA into the solution. After magnetic separation, the supernatant of the solution contains the released C-dots-cDNA which are quantified by fluorometry, best under excitation/emission wavelengths of 354/447 nm. Under the optimal conditions, the fluorescence intensity is proportional to the logarithm of the β-LG concentration in the 0.25 to 50 ng mL-1 range, with a 37 pg mL-1 detection limit. The method was successfully applied to the determination of β-LG in hypoallergenic formulations, and the results demonstrated that this assay is a promising tool in food quality control. Conceivably, it also provides the opportunity for detection of other analytes.
- Subjects
APTAMERS; MAGNETIC nanoparticles; LACTOGLOBULINS; MAGNETIC separation; COMPLEMENTARY DNA; FOOD quality; FOOD supply
- Publication
Microchimica Acta, 2018, Vol 185, Issue 1, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-017-2569-5