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- Title
Ultratrace voltammetric method for the detection of DNA sequence related to human immunodeficiency virus type 1.
- Authors
Wang, Ruibing; Xue, Chenghu; Gao, Mei; Qi, Honglan; Zhang, Chengxiao
- Abstract
n ultra-trace voltammetric method was developed for the determination of single strand DNA (ss-DNA) related to the human immunodeficiency virus type 1 (HIV-1). It is based on the signal amplification of carbon nanotubes loaded with silver nanoparticles and placed on a gold microelectrode. The capture ss-DNA (a 21-mer) possessing a thiol group at the 3′ end was self-assembled onto the surface of the gold microelectrode. It was then hybridized with target HIV-1 ss-DNA (a 42-mer) and further hybridized with the electrochemical probe (a 18-mer ss-DNA) tagged with multiwall carbon nanotubes and loaded with silver nanoparticles. The resulting formation of a DNA sandwich conjugate led to a strong electrochemical oxidation signal that was linearly proportional to the concentration of HIV-1 ss-DNA in the range from 1.0 to 100 pM. The detection limit was 0.5 pM (at an S/ N of 3). This was equivalent to 0.05 fmol of HIV-1 ss-DNA in a volume of 20 μL. The relative standard deviation was 4.0% at 1.0 pM ( n = 11). Non-complementary ss-DNA of HIV-1 ss-DNA was effectively discriminated. This work demonstrates that the employment of the microelectrode and a sandwich hybridization model is promising in terms of sensitive and selective electrochemical detection of DNA. [Figure not available: see fulltext.]
- Subjects
VOLTAMMETRY; NUCLEOTIDE sequence; DNA; HIV; CARBON nanotubes; NANOPARTICLES; MICROELECTRODES; NUCLEIC acid hybridization
- Publication
Microchimica Acta, 2011, Vol 172, Issue 3/4, p291
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-010-0490-2