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- Title
Comparison of four methods to quantify Equid herpesvirus 1 load by real-time polymerase chain reaction in nasal secretions of experimentally and naturally infected horses.
- Authors
Pusterla, Nicola; Hussey, Stephen B.; Mapes, Samantha; Leutenegger, Christian M.; Madigan, John E.; Ferraro, Gregory L.; Wilson, W. David; Lunn, D. Paul
- Abstract
The article compares the Equid herpesvirus 1 (EHV-1) glycoprotein B (gB) gene copies per 1 million nucleated nasal cells, per swab, per 1 microliter of extracted deoxyribonucleic acid (DNA), and per 1 nanogram of DNA in measuring the viral load of nasal secretions of naturally and infected horses using real-time polymerase chain reaction (PCR). Viral loads of artifically and naturally infected horses had positive correlation between the four methods. Nucleic acids from both groups at various time points were almost the same.
- Subjects
VIRAL load; HERPESVIRUSES; SECRETION; EQUINE herpesvirus diseases; GLYCOPROTEINS; DNA; NUCLEIC acids; POLYMERASE chain reaction
- Publication
Journal of Veterinary Diagnostic Investigation, 2009, Vol 21, Issue 6, p836
- ISSN
1040-6387
- Publication type
Article
- DOI
10.1177/104063870902100611