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- Title
Hematopoietic and nonhematopoietic potentials of Hoechst(low)/side population cells isolated from adult rat kidney.
- Authors
Iwatani, Hirotsugu; Ito, Takahito; Imai, Enyu; Matsuzaki, Yumi; Suzuki, Akira; Yamato, Masaya; Okabe, Masaru; Hori, Masatsugu
- Abstract
<bold>Background: </bold>Although the regenerative stem cell is expected to exist in many adult tissues, the cell contributing to the regeneration of the kidney remains unknown in its type and origin.<bold>Methods: </bold>In this study, we isolated cells that show low stain with a DNA-binding dye Hoechst 33342 (Hoechst(low) cells) from adult rat kidney, and investigated their differentiation potentials.<bold>Results: </bold>Hoechst(low) cells, generally termed side population cells, existed at a frequency of 0.03% to 0.1% in the cell suspension of the digested kidney. Analysis of the kidney-derived Hoechst(low) cells after bone marrow transplantation indicated that some of the cells were derived from bone marrow. When enhanced green fluorescent protein (EGFP)-labeled kidney-derived Hoechst(low) cells were intravenously transplanted into wild-type adult rats, EGFP(+) cells were not detected in the kidney, but EGFP(+) skeletal muscle, EGFP(+) hepatocytes and EGFP(+) bone marrow cells were observed. Even after the induction of the experimental glomerulonephritis and gentamicin-induced nephropathy that promote the differentiation of bone marrow-derived cells into repopulating mesangial cells and tubular component cells, respectively, EGFP(+) mesangial or tubular cells were not observed. Neither with an in vitro system, which we established to produce mesangial-like cells from crude bone marrow culture, did Hoechst(low) cells yield mesangial-like cells.<bold>Conclusion: </bold>These findings implicate that Hoechst(low) cells in the kidney may have potentials for hematopoietic and nonhematopoietic lineages, but are not stem cells for renal cells, especially mesangial and tubular cells.
- Subjects
PROTEIN metabolism; ANIMAL experimentation; BONE marrow; CELL differentiation; CELL separation; CELL transplantation; COMPARATIVE studies; GLOMERULONEPHRITIS; HEMATOPOIESIS; HEMATOPOIETIC stem cells; HETEROCYCLIC compounds; KIDNEYS; LIVER; RESEARCH methodology; MEDICAL cooperation; PROTEINS; RATS; RECOMBINANT proteins; RESEARCH; TRANSGENIC animals; EVALUATION research; SKELETAL muscle; FLUORESCENT dyes; IN vitro studies
- Publication
Kidney International, 2004, Vol 65, Issue 5, p1604
- ISSN
0085-2538
- Publication type
journal article
- DOI
10.1111/j.1523-1755.2004.00561.x