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- Title
Spindle and Chromosome Changes of Human MII Oocytes During Incubation After Slow Freezing/Fast Thawing Procedures.
- Authors
Gao, Shanshan; Yuan Li; Xuan Gao; Jingmei Hu; Huijun Yang; Zi-Jiang Chen
- Abstract
This experiment investigated the optimal time required for cryopreserved human oocytes to reform their spindles upon re-warming. Metaphase II oocytes were cryopreserved with a slow freezing method. Oocytes from each patient were randomly allocated into one of the 3 groups with different culture periods: 1, 3, or 5 hours, respectively, after thawing. Tubulin and chromosome configurations were visualized by confocal microscopy after immunostainings. By morphological assessment, 87.3% oocytes survived the process of freezing and thawing. Oocytes with normal spindle configuration increased significantly after 3 or 5 hours of incubation compared to those incubated for only 1 hour (P < 0.05). There were no differences in the chromosome configurations among the treatment groups (P > 0.05). This experiment demonstrated that cryopreserved human oocytes need a certain minimum period of incubation time (3 h) to recover their disrupted MII spindles and this information can be used in development of human IVF protocols with frozen oocytes.
- Subjects
OVUM; SPINDLE apparatus; TUBULINS; CHROMOSOMES; CRYOBIOLOGY; THAWING; CONFOCAL microscopy
- Publication
Reproductive Sciences, 2009, Vol 16, Issue 4, p391
- ISSN
1933-7191
- Publication type
Article
- DOI
10.1177/1933719108327590