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- Title
Curcumin affects apoptosis of colorectal cancer cells through ATF6‐mediated endoplasmic reticulum stress.
- Authors
Xu, Wei; Shen, Yu
- Abstract
Colorectal cancer (CRC) is the main cause of cancer‐associated death. Herein, we treated SW620 and HT‐29 CRC cells with different curcumin concentrations, followed by treatment with the half maximal inhibitory concentration (IC50) curcumin/endoplasmic reticulum stress (ERS) inhibitor 4‐phenyl butyric acid (4‐PBA)/activating transcription factor 6 (ATF6) interference plasmid (si‐ATF6). We detected cell proliferation/apoptosis, ATF6 cellular localization/nuclear translocation, ion concentration, ATF6 protein/apoptotic protein (Bax/Bcl‐2/Cleaved Caspase‐3) levels, and ERS‐related proteins (glucose‐regulated protein 78 [Grp78]/C/EBP homologous protein [CHOP]). We discovered inhibited cell proliferation/growth, enhanced cell apoptosis/(Bax/Bcl‐2) ratio/Cleaved Caspase‐3 levels/Ca2+ concentration in the cytoplasm/ERS‐related protein (Grp78/CHOP) levels, and activated ERS following treatment with IC50 curcumin. 4‐PBA partially reversed the inhibitory effect of curcumin on SW620 cells by restraining ERS. Curcumin stimulated ATF6 expression and its nuclear translocation to activate ERS. ATF6 silencing partly annulled the inhibitory effect of curcumin on SW620 cells. Our study explored the molecular mechanism of curcumin affecting CRC cell apoptosis through ATF6.
- Subjects
ENDOPLASMIC reticulum; COLORECTAL cancer; CURCUMIN; GLUCOSE-regulated proteins; CANCER cells
- Publication
Chemical Biology & Drug Design, 2024, Vol 103, Issue 1, p1
- ISSN
1747-0277
- Publication type
Article
- DOI
10.1111/cbdd.14433