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- Title
Inhibition of T cell-dependent antibody production by D-penicillamine.
- Authors
Petersen, J.
- Abstract
The effects of D-penicillamine (D-Pen) on the production of immunoglobulins (Ig) and anti-microbial antibodies (Ab) by human mononuclear cells (MNC) cultured <em>in vitro</em> were analyzed by hemolytic plaque forming cell (PFC) assays. Polyclonal Ig and Ab production, induced by pokeweed mitogen (PWM), was not affected by D-Pen in pharmacologically relevant concentrations, unless Cu2+ was added. Likewise, D-Pen + Cu2+, but not D-Pen alone, affected Polyclonal Ig production induced by Epstein-Barr virus. The production of interleukin-2 and B cell growth factor by phytohaemagglutinin-stimulated T cells was not inhibited by D-Pen; again D-Pen + Cu2+ markedly reduced the production of these cytokines. By contrast, D-Pen significantly reduced the antigen-induced antibody production without requirement of additional Cu2+. Addition of cytokine-containing supernatant to MNC treated with D-Pen + Cu2+ tended to increase the PWM-induced Ig responses, but had no effect on the antigen-induced Ab production of MNC cultured with D-Pen. Thus, the mechanisms by which D-Pen suppresses Polyclonal and antigen-induced B lymphocyte responses are different.
- Subjects
PENICILLAMINE; IMMUNOGLOBULINS; HEMOLYTIC plaque technique; DRUG therapy; EPSTEIN-Barr virus; INTERLEUKIN-2
- Publication
Allergy, 1987, Vol 42, Issue 1, p37
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/j.1398-9995.1987.tb02185.x