We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Simple Procedure for Cross-Linking Complementary Oligonucleotides.
- Authors
CHU, B.C.F.; ORGEL, LESLIE E.
- Abstract
A simple, efficient procedure for cross-linking two complementary oligonucleotides, which does not require chemical modification of either oligonucleotide, is described. One of the oligonucleotides is first converted to the 5′-phosphorothioate derivative with polynucleotide kinase. It is then incubated with its complement in the presence of 1 μ M trans-platinum(II)diammine dichloride. After overnight incubation, 40-50% cross-linking is observed. DNA synthesis by the Klenow fragment of Escherichia coli DNA polymerase I is blocked at the cross-linked site, resulting in the formation of truncated products. Potassium platinous chloride (K2PtCl4) and cis-platinum(II)diammine dichloride form cross-links less efficiently than the trans isomer.
- Publication
DNA & Cell Biology, 1990, Vol 9, Issue 1, p71
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.1990.9.71