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- Title
Sodium and Potassium ATPase of the Teleost Fish Catostomus commersoni.
- Authors
SCHÖNROCK, Christiane; MORLEY, Steven D.; OKAWARA, Yuji; LEDERIS, Karl; RICHTER, Dietmar
- Abstract
The α-subunit of a Na;⊕/K;⊕ ATPase has been cloned by analysing a λgt11 library constructed from polyA+ RNA from the hypothalamic region of the teleost fish Catostomus commersoni (white sucker). The cDN A clone consists of 3 853 bp and predicts a protein of 1027 amino-acid residues. Alignment of the sucker sequence with protein sequences previously published for α-subunits from various species reveals a high degree of homology throughout the entire sequence containing five potential sites for N-glycosylation, a phosphorylation site and a site for binding fluorescein 5'-isothiocyanate (FITC). A hydropathy profile predicts a secondary structure of the Na;⊕/K;⊕ ATPase α-subunit with at least eight membrane- spanning domains. Northern and southern blot analyses suggest the existence of two distinct Na;⊕/K;⊕ ATPase α-subunit genes in the sucker genome.
- Publication
Biological Chemistry, 1991, Vol 372, Issue 1, p279
- ISSN
1431-6730
- Publication type
Article