We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Biological characteristics of rat dorsal root ganglion cell and human vascular endothelial cell in mono- and co-culture.
- Authors
Yuan, Quan; Li, Jian-Jun; An, Chun-Hou; Sun, Li
- Abstract
This study aimed to evaluate the biological activity of rat dorsal root ganglion cell (DRGC) and human vascular endothelial cell (HMVEC) in mono- and co-culture. Expression levels of vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) mRNA were measured by quantitative real-time RT-PCR (qRT-PCR). Western blot analysis was used to identify VEGF and NGF protein expressions. Cell injury was assessed by measuring cell viability with methylthiazol tetrazolium (MTT) assay. The results showed that VEGF and NGF mRNA levels in the HMVEC+DRGC group were significantly higher than those in the DRGC and HMVEC groups (all p < 0.05). There were also greater increases in both VEGF and NGF protein expressions in the HMVEC+DRGC group than those in the DRGC and HMVEC groups (all p < 0.05). The results of MTT analysis revealed significant differences in cell viability among the HMVEC+DRGC group and the DRGC and HMVEC groups (all p < 0.05). In summary, our findings provide evidence that DRGC and HMVEC in co-culture may exhibit greater biological activity than DRGC in mono-culture.
- Subjects
LABORATORY rats; SPINAL ganglia; VASCULAR endothelial growth factors; CO-cultures; CELL survival; NERVE cell culture; PROTEIN expression; WESTERN immunoblotting
- Publication
Molecular Biology Reports, 2014, Vol 41, Issue 10, p6949
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-014-3581-z