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- Title
<italic>Rhodococcus psychrotolerans</italic> sp. nov., isolated from rhizosphere of <italic>Deschampsia antarctica</italic>.
- Authors
Silva, Leonardo Jose; Souza, Danilo Tosta; Genuario, Diego Bonaldo; Hoyos, Harold Alexander Vargas; Santos, Suikinai Nobre; Rosa, Luiz Henrique; Zucchi, Tiago Domingues; Melo, Itamar Soares
- Abstract
A novel actinobacterium, designated strain CMAA 1533T, was isolated from the rhizosphere of <italic>Deschampsia antarctica</italic> collected at King George Island, Antarctic Peninsula. Strain CMAA 1533T was found to grow over a wide range of temperatures (4-28 °C) and pH (4-10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533T belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus <italic>Rhodococcus</italic>. Sequence similarity calculations indicated that the novel strain is closely related to <italic>Rhodococcus degradans</italic> CCM 4446T, <italic>Rhodococcus erythropolis</italic> NBRC 15567T and <italic>Rhodococcus triatomae</italic> DSM 44892T (≤ 96.9%). The organism was found to contain <italic>meso</italic>-diaminopimelic acid, galactose and arabinose in whole cell hydrolysates. Its predominant isoprenologue was identified as MK-8(H2) and the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids were identified as Summed feature (C16:1 ω6c and/or C16:1 ω7c), C16:0, C18:1 ω9c and 10-methyl C18:0. The G+C content of genomic DNA was determined to be 65.5 mol%. Unlike the closely related type strains, CMAA 1533T can grow at 4 °C but not at 37 °C and was able to utilise adonitol and galactose as sole carbon sources. Based on phylogenetic, chemotaxonomic and physiological data, it is concluded that strain CMAA 1533T (= NRRL B-65465T = DSM 104532T) represents a new species of the genus <italic>Rhodococcus</italic>, for which the name <italic>Rhodococcus psychrotolerans</italic> sp. nov. is proposed.
- Subjects
RHIZOSPHERE; CULTURE media (Biology); RIBOSOMAL RNA genetics; NOCARDIACEAE; RHODOCOCCUS; GALACTOSE; ARABINOSE
- Publication
Antonie van Leeuwenhoek, 2018, Vol 111, Issue 4, p629
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-017-0983-7