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- Title
The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis.
- Authors
Peng, Xianjun; Zhang, Lexin; Zhang, Lixing; Liu, Zhujiang; Cheng, Liqin; Yang, Ying; Shen, Shihua; Chen, Shuangyan; Liu, Gongshe
- Abstract
S-Adenosyl-methionine decarboxylase (SAMDC) and dehydration responsive element-binding proteins (DREBs) can improve plant resistance to abiotic stresses. These proteins have been extensively studied, but the mechanism for transcriptional regulation of SAMDC remains unclear. In this paper, the LcSAMDC2 gene and its promoter were isolated from Leymus chinensis. Two DRE cis-elements were identified from the promoter of LcSAMDC2 and shown to bind with LcDREB2. Subcellular localization and yeast one-hybrid assay revealed that LcDREB2 is a transcription factor. An electrophoretic mobility shift assay (EMSA) showed that LcDREB2 can bind to the LcSAMDC2 promoter probe containing a DRE element. Over-expression of LcDREB2 in L. chinensis callus increased expression of LcSAMDC2. Co-expression of LcDREB2 and the promoter of LcSAMDC2 fused with GUS in tobacco activated GUS activity. These results indicate that LcSAMDC2 is the downstream gene of LcDREB2. In addition, transgenic expression of LcDREB2 and LcSAMDC2 in Arabidopsis can improve the salt stress tolerance of transgenic lines. These results indicate that LcDREB2 cooperating with LcSAMDC2 contributes to resistance to abiotic stress.
- Subjects
TRANSGENIC plants; GENETIC transcription in plants; CARRIER proteins; EFFECT of salt on plants; GENE expression
- Publication
Plant Cell, Tissue & Organ Culture, 2013, Vol 113, Issue 2, p245
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-012-0264-0