We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differential responses of <i>Mimusops elengi</i> and <i>Manilkara zapota</i> seeds and embryos to cryopreservation.
- Authors
Wen, Bin; Wang, Xiaofeng; Tan, Yunhong; Song, Songquan
- Abstract
Several dehydration protocols were evaluated for their ability to cryopreserve intact seeds and excised embryonic axes of Mimusops elengi and Manilkara zapota (Sapotaceae). Both interspecific and intraspecific variations in cryotolerance were found. M. zapota embryonic axes were more tolerant of cryopreservation than those of M. elengi, and showed higher desiccation tolerance, higher post-thawing survival and development, and a much wider range of moisture contents for cryopreservation. Maximum development rates were 94% and 27% for M. zapota and M. elengi, respectively. Intact seeds of both species tolerated desiccation to low moisture levels, but were sensitive to liquid nitrogen exposure, and cryopreserved seeds failed to germinate. Assessment of developing embryos excised from cryopreserved seeds associated nonviability of cotyledons and plumules with germination failure. Other structures survived at variable rates; most hypocotyls and radicles (up to 76% and 98% for M. elengi and M. zapota, respectively) were viable. The different cryotolerance between hypocotyls and cotyledons is a critical cause for failure in cryopreservation, contributing to the difficulty in developing protocols for such intermediate oily seeds.
- Subjects
SAPODILLA; CRYOPRESERVATION of organs, tissues, etc.; EASTERN meadowlark; DEHYDRATION; LIQUID nitrogen; COTYLEDONS
- Publication
In Vitro Cellular & Developmental Biology Plant, 2013, Vol 49, Issue 6, p717
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-013-9562-4