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- Title
Effect of Ischemia Duration on Autoantibody Response in Rats Undergoing Retinal Ischemia-Reperfusion.
- Authors
Joachim, Stephanie C.; Jehle, Thomas; Boehm, Nils; Gramlich, Oliver W.; Lagreze, Wolf A.; Pfeiffer, Norbert; Grus, Franz H.
- Abstract
Both the innate and the adaptive immune systems are involved in the pathogenic processes following ischemia-reperfusion injury. We analyzed the possible correlation between the duration of ischemia and autoantibody diversification in a model of ocular ischemia. Rats were subjected to 30, 45, or 90 min of ischemia, and retinal ganglion cell (RGC) density and antibody reactivity were analyzed via customized protein microarray slides. After ocular ischemia, significant alterations in antibody response were observed, while increasing exposure caused more severe RGC damage. Distinct antibody responses after ischemia were detected; these alterations comprised decreased reactivities against cyclophilin A and glyceraldehyde-3-phosphate dehydrogenase, possibly due to increased binding of circulating antibodies to debris material. Other antibodies, like those against α5β1-integrin or β2-adrenergic receptor, were upregulated after ischemia. Copyright © 2012 S. Karger AG, Basel
- Subjects
ISCHEMIA; AUTOANTIBODIES; RETINAL diseases; REPERFUSION; RETINAL ganglion cells; CYCLOPHILINS
- Publication
Ophthalmic Research, 2012, Vol 48, Issue 2, p67
- ISSN
0030-3747
- Publication type
Article
- DOI
10.1159/000335965