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- Title
Overexpression of Long Non-Coding RNA NNT-AS1 Correlates with Tumor Progression and Poor Prognosis in Osteosarcoma.
- Authors
Ye, Hui; Lin, Jinkuang; Yao, Xuedong; Li, Yizhong; Lin, Xiaobin; Lu, Hai
- Abstract
<bold><italic>Background/Aims:</italic></bold> Increasing evidence demonstrates that long non-coding RNAs (lncRNAs) play critical regulatory roles in cancers, including osteosarcoma. A previous study showed that Nicotinamide Nucleotide Transhydrogenase-antisense RNA1 (NNT-AS1) was aberrantly expressed in several types of cancer. However, the potential biological roles and regulatory mechanisms of NNT-AS1 in osteosarcoma progression remain unknown. <bold><italic>Methods:</italic></bold> Quantitative RT-PCR was performed to examine the expression of NNT-AS1 in human tissues and cells. The biological functions of NNT-AS1 were determined by CCK-8, colony formation, Flow cytometry and Transwell assays <italic>in vitro</italic>. A mouse xenograft model was performed to investigate the effect of NNT-AS1 on tumor growth <italic>in vivo</italic>. <bold><italic>Results:</italic></bold> In this study, we found the expression of NNT-AS1 was significantly increased in tumor tissues compared to adjacent normal tissues. Furthermore, upregulated NNT-AS1 expression predicted poor prognosis and was an independent and significant risk factor for osteosarcoma patient survival. Further experiments revealed that NNT-AS1 knockdown significantly inhibited cell proliferation by inducing cell cycle arrest and promoting apoptosis in osteosarcoma cells. Moreover, NNT-AS1 silencing suppressed cell migration and invasion <italic>in vitro</italic>. In a tumor xenograft model, knockdown of NNT-AS1 suppressed tumor growth of OS-732 cells <italic>in vivo</italic>. <bold><italic>Conclusions:</italic></bold> Taken together, these findings indicate that NNT-AS1 functions as an oncogene in osteosarcoma and could be a novel diagnostic and therapeutic target for osteosarcoma.
- Subjects
OSTEOSARCOMA; ONCOGENES; NON-coding RNA; GENETIC overexpression; APOPTOSIS; PROGNOSIS
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2018, Vol 45, Issue 5, p1904
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000487966