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- Title
Inhibition of acetate accumulation leads to enhanced production of ( R,R)-2,3-butanediol from glycerol in Escherichia coli.
- Authors
Shen, Xiaolin; Lin, Yuheng; Jain, Rachit; Yuan, Qipeng; Yan, Yajun
- Abstract
This work describes the production of ( R,R)-2,3-butanediol in Escherichia coli using glycerol by metabolic engineering approaches. The introduction of a synthetic pathway converting pyruvate to ( R,R)-2,3-butanediol into wild-type E. coli strain BW25113 led to the production of ( R,R)-2,3-butanediol at a titer of 3.54 g/l and a yield of 0.131 g product/g glycerol (26.7 % of theoretical maximum) with acetate (around 3.00 g/l) as the dominant by-product. We therefore evaluated the impacts of deleting the genes ackA or/and poxB that are responsible for the major by-product, acetate. This increased production of ( R,R)-2,3-butanediol to 9.54 g/l with a yield of 0.333 g product/g glycerol (68.0 % of theoretical maximum) in shake flask studies. The utilization of low-priced crude glycerol to produce value-added chemicals is of great significance to the economic viability of the biodiesel industry.
- Subjects
ACETATES; BUTANEDIOL manufacturing; GLYCERIN; ESCHERICHIA coli biotechnology; GENETIC engineering in microbiology; PYRUVATES
- Publication
Journal of Industrial Microbiology & Biotechnology, 2012, Vol 39, Issue 11, p1725
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-012-1171-4