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- Title
Enhancing Xylanase Production from Aspergillus tamarii Kita and Its Application in the Bioconversion of Agro-Industrial Residues into Fermentable Sugars Using Factorial Design.
- Authors
Salgado, Jose Carlos Santos; Heinen, Paulo Ricardo; Messias, Josana Maria; Oliveira-Monteiro, Lummy Maria; Cereia, Mariana; Rechia, Carem Gledes Vargas; Maller, Alexandre; Kadowaki, Marina Kimiko; Ward, Richard John; Polizeli, Maria de Lourdes Teixeira de Moraes
- Abstract
The endo-1,4-β-xylanases (EC 3.2.1.8) are the largest group of hydrolytic enzymes that degrade xylan, the major component of hemicelluloses, by catalyzing the hydrolysis of glycosidic bonds β-1,4 in this polymer, releasing xylooligosaccharides of different sizes. Xylanases have considerable potential in producing bread, animal feed, food, beverages, xylitol, and bioethanol. The fungus Aspergillus tamarii Kita produced xylanases in Adams' media supplemented with barley bagasse (brewer's spent grains), a by-product from brewery industries. The culture extract exhibited two xylanase activities in the zymogram, identified by mass spectrometry as glycosyl hydrolase (GH) families 10 and 11 (GH 10 and GH 11). The central composite design (CCD) showed excellent predictive capacity for xylanase production (23.083 U mL−1). Additionally, other enzyme activities took place during the submerged fermentation. Moreover, enzymatic saccharification based on a mixture design (MD) of three different lignocellulosic residues was helpful in the production of fermentable sugars by the A. tamarii Kita crude extract.
- Subjects
LIGNOCELLULOSE; XYLANASES; BREWER'S spent grain; FACTORIAL experiment designs; HYDROLASES; BIOCONVERSION; ASPERGILLUS; BREAD; WHEAT bran
- Publication
Fermentation (Basel), 2024, Vol 10, Issue 5, p241
- ISSN
2311-5637
- Publication type
Article
- DOI
10.3390/fermentation10050241