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- Title
高效液相色谱法测定化妆品中的盐酸二甲双胍.
- Authors
黄炜东
- Abstract
A high performance liquid chromatography method for determination of metformin hydrochloride in cosmetics was established. The samples were extracted by water under ultrasonic condition, and then separated on Thermo Acclaim 120 C18 (4.6 mm×250 mm, 5 μm) with methanol-10 mmol/L potassium dihydrogen phosphate solution (containing 1 mmol/L sodium dodecyl sulfate, pH=4.7) (50∶50) as mobile phase for isocratic elution. The flow rate was 1.0 mL/min, and the column temperature was 35 ℃. The detection was carried out by a diode array detector with the wavelength of 233 nm. Quantitative analysis was performed by the external standard method. The results show that there is a good linear relationship between the mass concentration of metformin hydrochloride and the peak area in the range of 0.05-2.0 mg/L (r=0.999 9). The detection limit is 0.30 mg/kg and the quantitative limit is 1.0 mg/kg. The recovery test was conducted by adding standard solution to the blank samples of different types of cosmetics. The recovery rates are in the range of 92.8%-110.0% with the relative standard deviations in the range of 1.96%-6.06% (n=6). This method has advantages such as simple pretreatment, high accuracy and almost little interference from the matrix. It is suitable for the determination of metformin hydrochloride in cosmetics.
- Subjects
HIGH performance liquid chromatography; POTASSIUM dihydrogen phosphate; SODIUM dodecyl sulfate; MICELLAR liquid chromatography; STANDARD deviations; DETECTION limit
- Publication
China Surfactant Detergent & Cosmetics, 2022, Vol 52, Issue 8, p863
- ISSN
1001-1803
- Publication type
Article
- DOI
10.3969/j.issn.1001-1803.2022.08.011