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- Title
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects.
- Authors
Jiang Zhu; Shiyi Wang; Cheng Wang; Zhi Wang; Gan Luo; Junhui Li; Yangyang Zhan; Dongbo Cai; Shouwen Chen
- Abstract
Microorganisms are important sources of various natural products that have been commercialized for human medicine and animal healthcare. Bacitracin is an important antibacterial natural product predominantly produced by Bacillus licheniformis and Bacillus subtilis, and it is characterized by a broad antimicrobial spectrum, strong activity and low resistance, thus bacitracin is extensively applied in animal feed and veterinary medicine industries. In recent years, various strategies have been proposed to improve bacitracin production. Herein, we systematically describe the regulation of bacitracin biosynthesis in genus Bacillus and its associated mechanism, to provide a theoretical basis for bacitracin overproduction. The metabolic engineering strategies applied for bacitracin production are explored, including improving substrate utilization, using an enlarged precursor amino acid pool, increasing ATP supply and NADPH generation, and engineering transcription regulators. We also present several approaches of fermentation process optimization to facilitate the industrial large-scale production of bacitracin. Finally, the challenges and prospects associated with microbial bacitracin synthesis are discussed to facilitate the establishment of high-yield and low-cost biological factories.
- Subjects
BACITRACIN; MICROBIOLOGICAL synthesis; MICROORGANISMS; ANTIBACTERIAL agents; FERMENTATION
- Publication
Synthetic & Systems Biotechnology, 2023, Vol 8, Issue 2, p314
- ISSN
2097-1206
- Publication type
Article
- DOI
10.1016/j.synbio.2023.03.009