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- Title
樟芝皿培菌丝提取物体外对SPCA-1肺癌细胞增殖的抑制作用.
- Authors
龙云凤; 吴莹莹; 贾薇; 杨海芮; 白旭; 张劲松; 杨焱; 张赫男; 栾军; 高玲; 王毅谦; 汪雯翰
- Abstract
Colony formation method, wound-healing method and alamarBlueTM cell viability assay were used to evaluate in vitro anti-tumor effects of petroleum ether extract and chloroform extract from dish-cultivated T. camphoratus mycelia on SPCA-1 non-small cell lung cancer cells. Flow cytometry Annexin V-FITC/PI double staining method was used to determine early apoptotic rate; flow cytometry 2', 7'-dichlorofluorescindiacetate(H2DCFDA) single staining method was used to determine ROS (reactive oxygen species) release; flow cytometry PI single staining method was applied to determine cell cycle changes; and ELISA method was used to determine expression of apoptosis-related proteins Caspase-3, p53 and PARP, hence to explore the anti-proliferation mechanism of extracts from dish-cultivated T. camphoratus mycelia on SPCA-1 lung cancer cells. The results showed that both petroleum ether extract and chloroform extract inhibited the colony formation, migration and proliferation of SPCA-1 cells. The activity of petroleum ether extract was significantly better than that of chloroform extract. The petroleum ether extract induced mitochondria-dependent early apoptosis by promoting the release of ROS from SPCA-1, which led to an increase in the activity of the pro-apoptotic proteins Caspase-3 and p53 and inhibition of PARP protein, thereby exerting its anti-tumor activity. The chloroform extract might exert its anti-tumor activity mainly by arresting SPCA-1 cells in S phase.
- Subjects
NON-small-cell lung carcinoma; ANNEXINS; P53 protein; CELL survival; CELL cycle; ADP-ribosyltransferases; FLOW cytometry
- Publication
Acta Edulis Fungi, 2019, Vol 26, Issue 2, p88
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2019.02.013