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- Title
A modified automated protocol for EBER in situ hybridization staining of xenograft tissue.
- Authors
AZIZ, N. A.; AHMAD, M.; SOO-BENG KHOO, A.
- Abstract
Introduction: Epstein-Barr virus encoded RNA (EBER) in situ hybridization (ISH) is a method for the identification of Epstein-Barr virus (EBV) infection in tissue sections. In our experience, the currently available automated EBER ISH staining protocol using the Bond-Max immunostainer is not suitable for xenograft tissue sample due to high background staining which impairs the interpretation of the staining. Methods: Here we describe a modified automated EBER ISH staining protocol for xenografts which involves the substitution of reagents for detecting the EBER probe. Results: This modified protocol significantly improved the quality and speed of the staining. Conclusion: The method described here will be useful for EBER ISH staining of xenograft tissue samples.
- Subjects
GENETICS of Epstein-Barr virus diseases; DIAGNOSTIC use of in-situ hybridization; IMMUNOSTAINING; XENOGRAFTS; RNA
- Publication
Journal of Morphological Sciences, 2018, Vol 35, Issue 3, p203
- ISSN
2177-0298
- Publication type
Article
- DOI
10.4322/jms.092815