In order to study the water-soluble chemical constituents of Swertia cincta. The 80% MeOH extract of S. cincta was successively partitioned with dichloromethane. And water soluble fraction was separated and purified by column chromatography and high performance liquid chromatography, in combination of the structure accredited by spectroscopic analysis. A new secoiridoid glycoside separated from the methanolic extract out of S. cincta, named swertiacincside C.