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- Title
Attenuated plasmodium sporozoite expressing MAGE-A3 induces antigen-specific CD8+ T cell response against lung cancer in mice.
- Authors
Dong Zhou; Hong Zheng; Quanxing Liu; Xiao Lu; Xufeng Deng; Li Jiang; Bing Hou; Yong Fu; Feng Zhu; Yan Ding; Wenyue Xu; Jigang Dai
- Abstract
Objective: Cancer vaccines that rely on tumor antigen-specific CD8+ T cell responses, are promising anti-cancer adjuvant immunotherapies. This study investigated whether genetically attenuated Plasmodium sporozoite (GAS) could be used as a novel vector to induce antigen-specific CD8+ T cell responses against lung cancer. Methods: We constructed GAS/MAGE-A3, a recombinant GAS engineered to express the lung cancer-specific antigen, melanomaassociated antigen 3 (MAGE-A3), and assessed its therapeutic effects against lung cancer. Results: Robust parasite-specific CD8αlowCD11ahigh and CD49dhighCD11ahigh CD4+ T cell responses as well as a MAGE-A3-specific CD8+ T cell response were induced in GAS/MAGE-A3-immunized mice. Adoptive transfer of GAS/MAGE-A3-induced CD8+ T cells from HLA-A2 transgenic mice into lung cancer-bearing nude mice inhibited tumor growth and prolonged survival. Conclusions: These findings demonstrate that GAS/MAGE-A3 induces a strong MAGE-A3-specific CD8+ T cell response against lung cancer in vivo, and indicate that GAS is a novel and efficacious antigen delivery vector for antitumor immunotherapy.
- Subjects
T cells; LUNG cancer; CANCER vaccines; TRANSGENIC mice; GAS engineering
- Publication
Cancer Biology & Medicine, 2019, Vol 16, Issue 2, p288
- ISSN
2095-3941
- Publication type
Article
- DOI
10.20892/j.issn.2095-3941.2018.0309