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- Title
Long‐term survival of full‐thickness corneal xenografts from α1,3‐galactosyltransferase gene‐knockout miniature pigs in non‐human primates.
- Authors
Yoon, Chang Ho; Choi, Se Hyun; Choi, Hyuk Jin; Lee, Hyun Ju; Kang, Hee Jung; Kim, Jong Min; Park, Chung‐Gyu; Choi, Kimyung; Kim, Hyunil; Ahn, Curie; Kim, Mee Kum
- Abstract
Background: We aimed to investigate (a) the long‐term survival of corneal grafts from α1,3‐galactosyltransferase gene‐knockout miniature (GTKOm) pigs in non‐human primates as a primary outcome and (b) the effect of anti‐CD20 antibody on the survival of corneal grafts from GTKOm pigs as a secondary outcome. Methods: Nine rhesus macaques undergoing full‐thickness corneal xenotransplantation using GTKOm pigs were systemically administered steroid, basiliximab, intravenous immunoglobulin, and tacrolimus with (CD20 group) or without (control group) anti‐CD20 antibody. Results: Graft survival was significantly longer (P =.008) in the CD20 group (>375, >187, >187, >83 days) than control group (165, 91, 72, 55, 37 days). When we compared the graft survival time between older (>7‐ month‐old) and younger (≤7‐month‐old) aged donor recipients, there was no significant difference. Activated B cells were lower in the CD20 group than control group (P =.026). Aqueous humor complement C3a was increased in the control group at last examination (P =.043) and was higher than that in the CD20 group (P =.014). Anti‐αGal IgG/M levels were unchanged in both groups. At last examination, anti‐non‐Gal IgG was increased in the control group alone (P =.013). Conclusions: The GTKOm pig corneal graft achieved long‐term survival when combined with anti‐CD20 antibody treatment. Inhibition of activated B cells and complement is imperative even when using GTKO pig corneas.
- Subjects
PRIMATES; SWINE; AQUEOUS humor; XENOGRAFTS; RHESUS monkeys
- Publication
Xenotransplantation, 2020, Vol 27, Issue 1, pN.PAG
- ISSN
0908-665X
- Publication type
Article
- DOI
10.1111/xen.12559