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- Title
Glucose levels observed in daily clinical practice induce endothelial dysfunction in the rabbit macro- and microcirculation.
- Authors
Gomes, Marília B.; Affonso, Felipe S.; Cailleaux, Solange; Almeida, Andressa L. F.; Pinto, Leonardo F. C.; Tibiriçá, Eduardo
- Abstract
We investigated whether different concentrations of elevated glucose – corresponding to levels observed in patients with type 2 diabetes under routine care (post-prandial mean and maximum values) and those used for diagnosing diabetes – induce impairment of vascular reactivity of the macro- and microcirculation in non-diabetic rabbits. Aortic rings and isolated perfused kidneys from normal rabbits were acutely exposed (3 h) to normal (5.5 m m) or high (7–25 m m) d-glucose concentrations. Vascular reactivity was evaluated with endothelium-dependent [acetylcholine (ACh) and bradykinin (BK)] and independent [sodium nitroprusside (SNP)] vasodilating agents. Endothelium-dependent relaxation of the thoracic aorta induced by ACh or BK was significantly attenuated after a 3-h exposure to high d-glucose (15–25 m m) but not after corresponding increased osmolarity with mannitol solutions. Relaxation induced by SNP (endothelium-independent) was not affected by high d-glucose concentrations. Moreover, endothelium-dependent but not independent vasodilation of the isolated rabbit kidney was also impaired after 3-h perfusion with high d-glucose (11.1–25 m m). Perfusion with mannitol solutions (15–25 m m) partially blunted endothelium-dependent renal vasodilation. It is concluded that acute hyperglycemia corresponding to post-prandial levels in patients with type 2 diabetes induces endothelial dysfunction of conduit vessels as well as of the renal circulation of non-diabetic rabbits.
- Subjects
HYPERGLYCEMIA; RENAL circulation; REACTIVITY (Chemistry); GLUCOSE; DIABETES; EPITHELIUM
- Publication
Fundamental & Clinical Pharmacology, 2004, Vol 18, Issue 3, p339
- ISSN
0767-3981
- Publication type
Article
- DOI
10.1111/j.1472-8206.2004.00248.x