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- Title
Establishment and characterization of cloned CD4<sup>-</sup> CD8<sup>-</sup> αβ-T cell receptor (TCR)-bearing autoreactive T cells from autoimmune NZB x NZW F<sub>1</sub> mice.
- Authors
Chen, Y.-C.; Ye, Y.-L.; Chiang, B.-L.
- Abstract
Murine models such as NZB/W 1, NZB.H-2bm12 and MRL.lpr/lpr mice have provided greater insight into the pathogenic mechanisms of lupus. To understand further the roles of T cells and cytokines in the pathogenesis of murine lupus, 11 cloned anti-DNA antibodies augmenting autoreactive T cell lines were derived from NZB/W F1 mice. All these autoreactive cells responded to syngeneic splenic cells and helped syngeneic B cells to produce anti-DNA antibodies, especially the IgG antibody. Ten out of 11 autoreactive T cell lines expressed neither CD4 nor CD8 cell surface markers on their surface. In addition, the cytokine production pattern of these autoreactive T cell lines was predominantly of type 0 (Th0) or type 2 T helper cells (Th2). To further investigate the role of accessory molecules in the activation of these autoreactive T ceil lines, expression of IL-2R and heal-stable antigen (HSA) on these autoreactive T cells was analysed. Results suggest that the HSA played a critical role in the activation and function of these double-negative cloned autoreactive T cells.
- Subjects
LYMPHOCYTES; CELL culture; CELLULAR immunity; IMMUNOGLOBULINS; CYTOKINES; IMMUNOREGULATION
- Publication
Clinical & Experimental Immunology, 1997, Vol 108, Issue 1, p52
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1046/j.1365-2249.1997.d01-971.x