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- Title
Interleukin-17 augments tumor necrosis factor-alpha-induced granulocyte and granulocyte/macrophage colony-stimulating factor release from human colonic myofibroblasts.
- Authors
Andoh A; Yasui H; Inatomi O; Zhang Z; Deguchi Y; Hata K; Araki Y; Tsujikawa T; Kitoh K; Kim-Mitsuyama S; Takayanagi A; Shimizu N; Fujiyama Y; Andoh, Akira; Yasui, Hirofumi; Inatomi, Osamu; Zhang, Zhuobin; Deguchi, Yasuyuki; Hata, Kazunori; Araki, Yoshio
- Abstract
<bold>Background: </bold>Interleukin (IL)-17 is a newly identified T-cell-specific cytokine. In this study, we investigated the effects of IL-17 on colony-stimulating factor (CSF) release in human colonic subepithelial myofibroblasts (SEMFs).<bold>Methods: </bold>CSF release and mRNA expression were determined by enzyme-linked immunosorbent assay (ELISA) and Northern blotting, respectively. Nuclear factor (NF)-kappaB- and activating protein (AP-1)-DNA binding activities were evaluated by electrophoretic gel mobility shift assays (EMSAs).<bold>Results: </bold>Unstimulated cells secreted a small amount of granulocyte G- and granulocyte/macrophage (GM)-CSF, and a considerable amount of M-CSF. IL-17 weakly enhanced G-CSF release, but did not affect GM- and M-CSF release. IL-17 selectively enhanced tumor necrosis factor (TNF)-alpha-induced G- and GM-CSF release. The combination of IL-17 plus TNF-alpha induced a marked increase in NF-kappaB- and AP-1-DNA binding activities. The adenovirus-mediated transfer of a stable form of IkappaBalpha and/or a dominant negative mutant of c-Jun markedly inhibited the IL-17 plus TNF-alpha-induced G- and GM-CSF mRNA expression. Furthermore, a stability study showed that IL-17 plus TNF-alpha markedly enhanced the stability of G- and GM-CSF mRNA.<bold>Conclusions: </bold>IL-17 augments TNF-alpha-induced G- and GM-CSF release via transcriptional and posttranscriptional mechanisms.
- Publication
Journal of Gastroenterology, 2005, Vol 40, Issue 8, p802
- ISSN
0944-1174
- Publication type
journal article
- DOI
10.1007/s00535-005-1632-x